Research Study Search

Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay [Read more]

Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay [Read more]

Release v1.2 of the Lens culinaris genome assembly for the canadian cultivar CDC Redberry has been made available by the University of Saskatchewan Pulse Crop Breeding program as PRE-RELEASE to aid collaborators in their research. It is protected under principles consistent with the Bermuda and

This project contains phenotypes taken opportunistically during RIL Development of LR-03. Only orphan data taken during RIL development should be included in this project It should not include data taken as part of a graduate student project or data taken to answer a specific question

This project contains phenotypes taken opportunistically during RIL Development of LR-06. Only orphan data taken during RIL development should be included in this project It should not include data taken as part of a graduate student project or data taken to answer a specific question

This experiment was conducted to detect differential expression patterns between dormant and non-dormant seeds. For this, RNA was extracted from one wild parent and two cultivated parents. For each, the RNA was extracted at four developmental stages, separating the embryo and the seed coat tissue, [Read more]

Through the study of 10 genotypes from 6 Lens species, in combination with 4 Rhizobium strains from 3 species, the specificity at the accession × strain level will be determined. Infectiveness and effectiveness of this multiple combinations will be available, setting the groundwork for a better [Read more]

Through evaluating 8 lentil genotypes in 2 different macro-environments, based on phenological parameters, seed characteristics, nutritional profile (carotenoids), weather during the growing season and Slick test (final products), the causal effects on red colour stability of red lentil flour, will [Read more]

LensTwo intra and interspecific RIL populations  LR-70 (Eston L. culinaris × IG 72623 L. odemensis) and LR-93 (CDC Redberry L. culinaris × CDC Greenstar L.culinaris) were evaluated to assess the variation on B vitamin concentration cross genotypes and seed tissue types (cotyledon and whole [Read more]

Surveying existing B-vitamin [Read more]

In 2015, we embarked upon an ambitious genomics project Application of Genomic Innovation in the Lentil Economy (AGILE) that has delivered the first genome long-read assembly for CDC Redberry (genus Lens), assemblies for 2 wild lentil species, and molecular markers for phenology. This was a start [Read more]

# Amino acid score: The amino acid compositions of the lentil samples were used to estimate the Amino Acid Score (AAS) as [mg of amino acid in 1 g of test protein/mg of amino acid in requirement pattern]×100 (FAO/WHO, 1991). Different age-categories scoring patterns were used: (a) pre-school [Read more]

Seed dormancy is one barrier hindering the use of wild lentil species in breeding programs and associated scientific study. In this set of experiments, we studied the possibility of replacing manual seed scarification methods of breaking seed dormancy with chemical seed treatmentsEndogenous ABA is [Read more]

 Growth habit is one of the most significant agronomic traits involved in the domestication process. Growth habit in lentil breeding encompasses alterations related to plant structure affecting production and yield stability. This population, IG 72643 (L. orientalis) x 3339-3 (L. culinaris [Read more]

"Milling qualities are key traits for the red lentil (Lens culinaris Medik.) industry as price is largely determined by dhal recovery yield. Dhal milling involves removal of the seed coat and splitting of the cotyledon to produce either splits or footballs (cotyledons still attached). The objectives [Read more]

    Today, superior Canadian lentil cultivars are expected to grow well in our northern growing conditions while being resilient to various abiotic and biotic stresses.  The breeders achieve this by using diverse materials in their crosses, but need to ensure that offspring from these [Read more]

Measuring biomass (above ground plant material biomass) in 6 varieties (ILL 7716, CDC Asterix, PI 490288 LSP, CDC Redcoat, CDC Cherry, ILL 9888) with a sample (micro plot -3 replicates) taken every two weeks. To correlate biomass with a volume estimate from UAV images [Read more]

This project contains phenotypes taken opportunistically during RIL Development of LR-68. Only orphan data taken during RIL development should be included in this project It should not include data taken as part of a graduate student project or data taken to answer a specific question [Read more]

Growth habit is one of the most significant agronomic traits involved in the domestication process. Growth habit in lentil breeding encompasses alterations related to plant structure affecting production and yield stability. This population, Eston (L. culinaris) x IG 72623 (L. odemensis), is being [Read more]

A diverse collection of lentil accessions is being phenotyped for days to flower and screened against potential flowering time genes Identified by other researcher groups. In addition to the confirmation and the development of markers useful for the prediction of flowering time in northern temperate [Read more]

A major priority for most plant breeders is to add new genotypes in their breeding programs. For that, they use a variety of breeding techniques, and often the times, they take new genotypes from a completely new environment. However, the introduction of new genotypes can be tiresome work [Read more]

Lentil recombinant inbred lines (LR-86) derived from a cross between Lupa # 7 (L. culinaris) x BGE016880 (L. orientalis) were evaluated in five replications in 2016 in the field at the Crop Science Field Lab of the University of Saskatchewan. Days to flowering, days to maturity, plant height at [Read more]

 Light is essential for plant growth and development. Green plants selectively absorb blue and red wavelengths of the light, which resulted in a decrease in red/far-red (R/FR) ratio in the surrounding environment. The reduction is sensed by the plants, thereby signaling the presence of [Read more]

This research project aims to efficiently expand the genetic diversity of the Canadian lentil breeding program. Targeted-exome resequencing of the AGILE lentil diversity panel will provide the information needed to determine which germplasm can provide that genetic diversity, as well as, be used to [Read more]

Cultivated lentil is a quantitative long day plant. In-season temperature and daylength varies considerably among three main growing regions, which are northern temperate regions (SK, North Dakota), Mediterranean climates (Turkey, Spain, Morocco), and South Asian (India, Bangladesh, Nepal). [Read more]

Data:Throughout the AGILE project, KnowPulse has acted as the official data warehouse for the wealth of data generated. These data can be accessed through the specific research projects linked to on the main AGILE page, as well as, within tools and searches accessible from the frontpage. As data are [Read more]

Our first GE3LS objective in AGILE was to better understand how and why producers adopt new lentil varieties. We attempted to uncover the institutional, community and personal characteristics and processes that facilitate uptake and use of new crop varieties.Our original research target was the [Read more]

Our second GE3LS goal was the examination of impacts to industry with respect to changes in the current royalty system for plant breeders. The adoption of UPOV ‘91 will change the way that royalties are collected and how much of the royalties are fed back into Canadian breeding programs. This [Read more]

Development of improved lentil cultivars well-adapted to the local environment is an on-going process in the breeding program and is critical for long-term genetic gain. Recent climate instability adds another layer of complexity to breeding efforts. Continued genetic improvement of lentil will, [Read more]

    Nitrogen fixation is a symbiotic relation between legumes and Rhizobium that allows the bacteria to convert atmospheric nitrogen to other molecules (like ammonia) for the plant, and the plant to provide the bacteria with carbohydrates in exchange.  We now know that this process [Read more]

    We have many different types of lentil grown in over 50 countries around the world.  The timing to grow the crop is different depending on where you are.  In Canada, lentil is sown in May and harvested in August.  Whereas in Nepal, lentil is sown in October and harvested [Read more]

    Lentil breeders sometimes use exotic germplasm to broaden the genetic base and introduce desirable traits to elite cultivars. However, offsprings from these wide crosses often adapt poorly in the short growing season of western Canada. Identifying regions in the lentil genome that [Read more]

This is an international project funded by the Global Crop Diversity Trust (https://www.croptrust.org/) aimed at evaluating cultivated x wild lentil introgression lines for multiple traits in multiple environments with a view to identifying promising lines with tolerance to multiple stresses.Use of [Read more]

A number of KASP markers were developed based on the genotypes identified under the Lentil 454 Sequencing Project. An initial set were used for validation of the SNP calling before developing the Illumina Golden Gate Assay (Lc1536).  An additional 350 KASP primers were then designed for the [Read more]

Lentil is a very important crop for the Saskatchewan economy.  In contrast to the industry success, lentil itself is considered an orphan crop from a genomics perspective.  Until recently, very few molecular genetic and genomic resources existed for lentil, partly due to its relatively [Read more]

Lentil is a self-pollinating annual cool season legume with seed coat colours that can be clear, green, tan, gray, brown, or black. The seed coat is a good source for phenolic compounds, which can have health benefits such as anti-oxidant, anti-tumor, and anti-heart disease properties. Two [Read more]

An Illumina Golden Gate array was developed using SNPs identified as part of the Lentil 454 Sequencing & Genotyping Project.   454 pyrosequencing technology was used for targeted 3′-cDNA transcript profiling for the nine L. culinaris genotypes and two L.  [Read more]

Eleven germplasm lines were chosen for this project: CDC Milestone, CDC Redberry, CDC Robin, Eston-A, ILL 5588, ILL 8006, LC 860230-3T, L01-827A, PI 320937, IG 72815, 964a-46. Tissue was collected from multiple plants at various developmental stages for RNA extraction which led to the generation of [Read more]

Lentil is an economically important pulse crop for Canada produced mainly for the export market. In conventional breeding programs, several segregating generations must be grown in order to reach a certain level of homozygosity that allows the selection of traits of interest. In contrast, double- [Read more]

Saskatchewan is by far the largest lentil exporter in the world and in some years, the largest producer.  Lentil anthracnose is currently the most important lentil disease in Saskatchewan. In a project about to be completed on molecular aspects of this pathogen, we generated an extensive [Read more]

A set of 1107 legume cross species orthologous sequences (COS) were amplified   from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815).  Sequences were aligned and SNPs identified.  A subset of 110 KASP assays were designed for use in L. culinaris.& [Read more]

Lentil has been grown commercially in western Canada since 1970. Ascochyta lentis, the causal agent of ascochyta blight of lentil is established as one of the most economically important diseases of lentil in Western Canada. Resistance breeding is important for developing a durable and sustainable [Read more]

Stemphylium blight caused by the fungal pathogen Stemphylium botryosum is a lentil disease that has become more prominent in Saskatchewan in recent years. The disease is not well studied under our growing conditions, and information is sketchy on optimal conditions for serious outbreaks of [Read more]