Lentil UC Davis COS Genotyping

A set of 1107 legume cross species orthologous sequences (COS) were amplified   from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815).  Sequences were aligned and SNPs identified.  A subset of 110 KASP assays were designed for use in L. culinaris.  An Illumina GoldenGate array of 768 SNPs was designed for use in L. ervoides or interspecies hybrid populations between Lc and Le.

A map of L.ervoides, consisting of 377 SNP markers spanning seven linkage groups was developed and used to compare the genetic structure of Le with Medicago.  There was extensive collinearity between the 2 genomes.