Lentil 454 Sequencing & SNP Discovery Project

Eleven germplasm lines were chosen for this project: CDC Milestone, CDC Redberry, CDC Robin, Eston-A, ILL 5588, ILL 8006, LC 860230-3T, L01-827A, PI 320937, IG 72815, 964a-46. Tissue was collected from multiple plants at various developmental stages for RNA extraction which led to the generation of 3'-anchored cDNA libraries using the method described in Parkin et al. (Genome 2010, 53:929–938). Each line was sequenced using the Roche 454 Titanium sequencing protocol. Sequencing reads were assembled into contigs using the NGen assembler resulting in 27,921 CDC Redberry contigs. Contigs from the other 10 lines were compared to CDC Redberry and loci which were polymorphic between CDC Redberry and at least one other line were identified resulting in 44,942 SNPs.