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Nucleotide Query
Nucleotide to Protein BLAST (blastx)
Request a New BLAST
Enter
Nucleotide
Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Enter query sequence(s) in the text area.
Choose Search Target
Choose from one of the
protein
BLAST databases listed below.
Protein
BLAST Databases:
Select a Dataset
Cicer arietinum: CDC Frontier Genome 1.0 Protein Dataset
Cicer arietinum: ICC 4958 Genome 2.0 Protein Dataset
Glycine max: Williams 82 Genome 4.0 Protein Dataset
Phaseolus vulgaris: G19833 Genome 2.0 Protein Dataset
Pisum sativum: Caméor 1a Protein Dataset
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
1000
5000
10000
20000
Select the maximum number of aligned sequences to display
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
The length of the seed that initiates an alignment
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Assigns a score for aligning pairs of residues, and determines overall alignment score..
Gap Costs:
Existence: 11 Extension: 2
Existence: 10 Extension: 2
Existence: 9 Extension: 2
Existence: 8 Extension: 2
Existence: 7 Extension: 2
Existence: 6 Extension: 2
Existence: 13 Extension: 1
Existence: 12 Extension: 1
Existence: 11 Extension: 1
Existence: 10 Extension: 1
Existence: 9 Extension: 1
Cost to create and extend a gap in an alignment.
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Bioinformatic Tools
BLAST
Nucleotide Query
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Cicer arietinum (Kabuli), CDC Frontier v1.0
Cicer arietinum (Desi), ICC4958 v2.0
Colletotrichum lentis, CT30 v1.0
Common Bean, G19833 v2.1
Glycine max, Williams 82 v4.0
Lens culinaris, CDC Redberry v1.2
Medicago truncatula, A17 v4.0
Pisum sativum, Caméor v1a
Vigna unguiculata, IT97K-499-3 v1.2
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