AGILE Activity 2: Phenotyping in three major lentil production macro-environments

AGILE Activity 2: Phenotyping in three major lentil production macro-environments

Cultivated lentil is a quantitative long day plant. In-season temperature and daylength varies considerably among three main growing regions, which are northern temperate regions (SK, North Dakota), Mediterranean climates (Turkey, Spain, Morocco), and South Asian (India, Bangladesh, Nepal). Different temperature and daylength among the regions lead to different plant life cycle. In northern temperate regions, sowing is between April to May when days are lengthening and temperatures rising. Adapted germplasm will typically flower in early July, about 1-2 weeks after the summer solstice. In mediterranean climates, sowing is usually between November to December as days are shortening and temperatures are mild. Flowering of adapted germplasm in these regions is typically delayed until mid-March when days lengthen and temperatures start to rise. In South Asia, the entire plant life cycle occurs during daylengths that are below the threshold daylength for induction of flowering for either mediterranean or northern temperate adaptation. When grown in Canada, un-adapted germplasm typically flowers at inappropriate times and result in yield penalty.

 While daylength responses are fairly predictable, the interaction between daylength response and temperature is not. In the project 2a, we hypothesize that there will be variation in response to temperature leading up to flowering. Also, we hypothesize that a number of lines that are adapted to one region will respond correctly to light and temperate in other regions.

Seed of the lentil diversity panel will be increased in Saskatoon and sent to three locations in each macro-environment for field phenotyping for a minimum of two seasons. Phenotypic data will be collected from northern temperate locations and where possible, from the other locations. Genetic control and heritability of the traits that are segregating will be determined through the statistical analysis.

              Result of AGILE Activity 2 will deliver comprehensive dataset of phenological data from contrasting environments for 350 diverse lentil lines, as well as to determine the genetic control of key phenological traits for lentil. Additionally, phenotypic data can be used for marker association analyses in other research experiments. 


Published Materials
  1. Neupane S, Wright DM, Martinez RO, Butler J, Weller JL, Bett KE. Focusing the GWAS Lens on days to flower using latent variable phenotypes derived from global multienvironment trials.. The plant genome. 2023 Mar; 16(1):e20269.
  2. Haile TA, Heidecker T, Wright D, Neupane S, Ramsay L, Vandenberg A, Bett KE. Genomic selection for lentil breeding: Empirical evidence.. The plant genome. 2020 03; 13(1):e20002.
  3. Neupane S, Dhakal R, Wright DM, Shrestha DK, Dhakal B, Bett KE. Strategic Identification of New Genetic Diversity to Expand Lentil (Lens culinaris Medik.) Production (Using Nepal as an Example)..Agronomy.2021; 11:11101933.
  4. Wright, DM, Neupane, S, Heidecker, T, et al. Understanding photothermal interactions will help expand production range and increase genetic diversity of lentil (Lens culinaris Medik.). Plants, People, Planet. 2021; 3: 171– 181.
Phenotype Experiment
Phenotypic Traits
Focuses on phenology traits
Location(s) of Field Trials
Sutherland Canada, Jessore Bangladesh, Marchouche Morocco, Central Ferry USA, Bhopal India, Bardiya Nepal, Rosthern Canada, Metaponto Italy

The phenotypic data is best summarized in a trait distribution chart. To see the summary for your trait of interest, select it from the drop-down below. If the trait was measured with multiple methods in this experiment, you will see each method displayed in it's own chart.

Select the trait you are interested in.
Associated Datasets
The following datasets are related to the current experiment:

Phenology Official Data
: an Excel file containing the final, cleaned dataset for Days till Plants Emerge (Days till 10% Plants/plot have emerged; days), Days till Plants have One Open Flower; R1 (Days till 10% plants/plot at R1; days), Days till Plants have fully Swollen Pods; R5 (Days till 10% plants/plot at R5; days), Days till Plants have 1/2 of their Pods Mature; R7 (Days till 10% Plants/plot at R7; days). An R-driven application for exploring the phenotypic data associated with this experiment.

Github/derekmichaelwright/AGILE_LDP_Phenology: The linked github repository contains the raw data and various figures describing it.

Exome Capture SNP dataset: Takes you to KnowPulse's VCF Bulk Export where you can filter and download the SNP data for the AGILE LDP germplasm in various formats, including VCF.