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Protein Query
Protein to Nucleotide BLAST (tblastn)
Request a New BLAST
Enter
Protein
Query Sequence
Enter one or more queries in the top text box or use the browse button to upload a file from your local disk. The file may contain a single sequence or a list of sequences. In both cases, the data must be in
FASTA format
.
Show an Example Sequence
Enter FASTA sequence(s)
Enter query sequence(s) in the text area.
Choose Search Target
Choose from one of the
nucleotide
BLAST databases listed below.
Nucleotide
BLAST Databases:
Select a Dataset
Cicer arietinum: CDC Frontier Genome Build 1.0
Cicer arietinum: Frontier 454 Contigs
Cicer arietinum: ICC 4958 Genome Build 2.0
Glycine max: DFCI Indices version 16
Glycine max: Genome 2.0
Glycine max: Williams 82 Genome 4.0
Lens culinaris: CDC Redberry 454 contigs
Lens culinaris: CDC Redberry Genome 1.2
Lens culinaris: NAPGEN Contigs and ESTs
Lotus japonicus: DFCI Indices version 6
Medicago truncatula: A17 Genes v3.5
Medicago truncatula: A17 Genome Build 4
Medicago truncatula: DFCI Indices version 9
Phaseolus coccineus: DFCI Indices version 1
Phaseolus vulgaris: 2793CBB 454 contigs
Phaseolus vulgaris: DFCI Indices version 4
Phaseolus vulgaris: G19833 Genome 2.1
Phaseolus vulgaris: Genome Build 1.0
Pisum sativum: Bronco 454 contigs
Pisum sativum: Caméor genome build 1a
Vicia faba: NCBI ESTs (2015Mar3)
Vigna unguiculata: DFCI Indices version 1
Advanced Options
General parameters
Max target sequences:
10
50
100
250
500
1000
5000
10000
20000
Select the maximum number of aligned sequences to display
e-Value (Expected Threshold)
Expected number of chance matches in a random model. This number should be give in a decimal format.
More Information
|
Expect value vedio tutorial
Word size:
3
6
The length of the seed that initiates an alignment
Scoring parameters
Matrix
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Assigns a score for aligning pairs of residues, and determines overall alignment score.
Gap Costs:
Existence: 11 Extension: 2
Existence: 10 Extension: 2
Existence: 9 Extension: 2
Existence: 8 Extension: 2
Existence: 7 Extension: 2
Existence: 6 Extension: 2
Existence: 13 Extension: 1
Existence: 12 Extension: 1
Existence: 11 Extension: 1
Existence: 10 Extension: 1
Existence: 9 Extension: 1
Cost to create and extend a gap in an alignment.
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Cicer arietinum (Kabuli), CDC Frontier v1.0
Cicer arietinum (Desi), ICC4958 v2.0
Colletotrichum lentis, CT30 v1.0
Common Bean, G19833 v2.1
Glycine max, Williams 82 v4.0
Lens culinaris, CDC Redberry v1.2
Medicago truncatula, A17 v4.0
Pisum sativum, Caméor v1a
Vigna unguiculata, IT97K-499-3 v1.2
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