Cicer arietinum 454 Sequencing & SNP Discovery Project

Eight germplasm were chosen for this project: Amit, CDC Frontier, CDC Xena, Cr5-10 ICC12512-1, ICCV96029, ILWC 118, Y9563-28. Tissue was collected from multiple plants at various developmental stages for RNA extraction which led to the generation of 3'-anchored cDNA libraries using the method described in Parkin et al., 2010. Each line was sequenced using the Roche 454 Titanium sequencing protocol. Sequencing reads were aligned directly to the current Chickpea genomic build (unreleased) using GMap. Then loci which were polymorphic between at least two of the lines were identified.