Red Lentil flour colour intensity: 8 Lentil germplasm using colourimeter and slick test in 2024

Overview

Objectives

Evaluate how stable the red colour of lentil flour samples is when hydrated and baked.
Determine whether the intensity of red colour for a dehulled lentil seed is predictive of the intensity of the baked flour sample obtained from that seed.

Parent Research Study

Effect of genotype and environment on colour stability of lentils through processing to dehulled seeds, flour and food products

Experiment Summary

This experiment evaluates the intensity of red colour in Lentil flour samples and the stability of this colour intensity through the process of hydrating and baking. The flour samples were obtained by milling dehulled seeds from Lentil Red cotyledon intensity: Lentil germplasm over 8 site-years in 2 macroenvironments from 2022-2023 using BELT. CIE L*, a* and b* colour scores were obtained for pressed flour for each replicate from the linked field study. Then composite flour samples obtained by combining field replicates, were evaluated using the Pekar Color Test (also known as slick test) for CIE L*, a* and b* colour scores when hydrated and when baked. Both the A* and B* scores were used to evaluate redness.

Germplasm

Germplasm Genus	Lens
Germplasm Scientific Name	Lens culinaris
Germplasm Collection	W6 27760, CDC KR-1, CDC Redberry, CDC Robin, CN 105604, ILL 213, PI 612875, ITACA

Phenomics

Key Measurements

CIE L*, a* and b* colour scores

Experimental Design

Experimental Site Locations	Sutherland, Saskatchewan, Canada Rosthern, Saskatchewan, Canada Osimo, Ancona, Italy Montemurro, Potenza, Italy
Timepoints	2022 2023
Biochemical Technique	Pekar Color Test (Slick test)

Experimental Design

The flour samples were obtained by milling dehulled seeds with a cyclone sample mill (Udy Corporation, CO, USA) from Lentil Red cotyledon intensity: Lentil germplasm over 8 site-years in 2 macroenvironments from 2022-2023 using BELT. Seeds from a single plot were used to create a single flour sample for the pressed flour measurements.
CIE L*, a* and b* colour scores were obtained using the Nix Spectro 2 (Nix Sensor Ltd., Hamilton ON, Canada) for pressed flour samples. Specifically, Illuminant C and an observer angle of 2 degrees were used. A white normalization scan was conducted with a ceramic reference tile prior to measuring samples each time the NIX was turned on.
We combined repetitions from each site year generating composite mixes which were used for the Pekar test (slick test). Approx. 5g of milled lentil flour was spread across a metal plate and slowly immersed in water at an angle of 30° for five seconds. CIE L*, a* and b* colour scores of the wet flour were obtained with the Minolta Chroma Meter CR-400 (Minolta Camera Co., Osaka, Japan) using the standard Illuminant C and observer angle of 2 degrees. These same hydrated samples were then baked at 135 degrees Celcius for 5 minutes and then CIE L*, a* and b* colour scores were obtained using the same settings as with the hydrated samples.

Attribution

Researchers

Data Collector	Ana Vargas Sarahi Viscarra
Data Custodian	Kirstin E Bett Ana Vargas
Data Curator	Lacey-Anne Sanderson Ruobin Liu

Associated Datasets

Pressed Flour CIE L*, a*, and b* scores (Excel, TSV)
This dataset consists of the CIE L*, a*, and b* scores for dry pressed flour samples. All 8 site-years from the linked field experiment are represented for 8 Lentil genotypes with the field biological replicates analyzed separately. The mean scores in a single row are obtained from a single scan where two technical additional repetitions were added to every scan using the multi-point averaging technique in the Nix Toolkit App.
Hydrated and Baked Flour CIE L*, a*, and b* scores (Excel, TSV)
This dataset consists of the CIE L*, a*, and b* scores for hydrated and baked flour samples obtained by performing a Pekar (Slick) Test. All 8 site-years from the linked field experiment are represented for 8 Lentil genotypes with biological replicates within a site year being combined. The scores in a single row are obtained from a single scan with the Minolta Chroma Meter CR-400 on.

Funding

Research Organization

Funding Grant