Lentil Red cotyledon intensity: Lentil germplasm over 8 site-years in 2 macroenvironments from 2022-2023 using BELT

Overview
Objectives
  • Grow Red Lentil genotypes in two major growing regions in order to obtain seed for downstream red colour stability and intensity experiments.

  • Evaluate the variation in colour across different environments and determine the baseline CIE L*, a* and b* colour scores for dehulled seeds of these genotypes.

Parent Research Study
Effect of genotype and environment on colour stability of lentils through processing to dehulled seeds, flour and food products
Experiment Summary

This experiment evaluates 8 lentil genotypes grown in 2 different macro-environments (Canada and Italy) for the intensity of red colour in dehulled seeds. The genotypes were assessed for phenological parameters while in the field including days till emergence, flowering and maturity as well as yield. Then the harvested seeds were dehulled and imaged using the BELT system to assess red colour using the lab colour space. These data will be used to assess the baseline redness of these varieties before the seed is used in subsequent experiments to analyze the stability of that red colour.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
Lens culinaris
Germplasm Collection
W6 27760, CDC KR-1, CDC Redberry, CDC Robin, CN 105604, ILL 213, PI 612875, ITACA
Phenomics
Key Measurements
  • Thousand Seed Weight (g), Total Plot Yield (g)
  • Days till 50% of the plot have hypocotyl visible (days), Seedling count whole plot (count)
  • Days till 50% of the plot have flowered (days), Days till 50% of the plot reaching maturity (days)
  • CIE L*, a* and b* colour scores
Experimental Design
Experimental Site Locations
  • Sutherland, Saskatchewan, Canada
  • Rosthern, Saskatchewan, Canada
  • Osimo, Ancona, Italy
  • Montemurro, Potenza, Italy
Timepoints
  • 2022
  • 2023
Experimental Design

  • Field trials were arranged in a Randomized Complete Block Design (RCBD) with the exact number of replicates depending on the resources available in that specific site-year. In 2022, plots (three rows 4 m x 0.5 m) were seeded in Rosthern (N 52.7418 E -106.23) and Sutherland (N 52.1725 E -106.505), SK, Canada in four replications, and Osimo, Italy (N 43.4601 E 13.4961) in three replications. In 2023 plots were seeded in Rosthern and Sutherland in eight replications and Osimo (N 43.4601 E 13.4961), and Montemurro (N 40.3022 E 15.9981), Italy, in three replications.

  • Trials in Canada were grown during the typical growing season – from May to August, in plots of 3 m2, 3 rows per plot, with inter-row spacing of 30 cm, a row length of 3 m, and a target population of 540 plants per plot. The trial in Italy was grown during February to August in plots of 5 m2, 5 rows per plot, with an inter-row spacing of 30 cm, a row length of 3 m, and a target population of 900 plants per plot.

Attribution
Researchers
Data Collector
Data Custodian
Data Curator
Associated Datasets

Field Phenology Dataset (Excel, TSV)

This dataset records phenological observations of this field trial, specifically, emergence, flowering, maturity, yield and lodging. These observations were taken for all 8 siteyears with each site-year containing 3-8 replicates of the 8 genotypes with the exact number of replicates depending on the resources available in that specific siteyear (see experimental design).

Funding
Research Organization
Funding Grant
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
  • Kirstin E Bett
  • Albert Vandenberg
University of Saskatchewan

2019-2023

Mitacs Accelerate Grant (IT29802)
Kirstin E Bett
University of Saskatchewan

2022-2024