Using a Nix Spectro2 to differentiate milled flour of red lentil genotypes

Overview
Objectives

Test the accuracy of the Nix Spectro 2 on processed flour products (specifically lentil) to determine if it comparable to the HunterLab colorimeter which is the current industry standard.

Parent Research Study
Effect of genotype and environment on colour stability of lentils through processing to dehulled seeds, flour and food products
Experiment Summary

We validated the use of the Nix Spectro 2 to assess food color by observing the results obtained with the Nix to those generated by the HunterLab (an industry standard). The a* and b* Nix scores were comparable to those obtained with the HunterLab (r2 = 0.9-0.95). L* scores had lower correlation values: 0.61 and 0.58 when comparing HunterLab (C) with Nix (D50) and Nix (C), respectively. Lower L* correlations could be a result of the influence of direct light exposure during HunterLab scans.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
Lens culinaris
Germplasm Collection
W6 27760, CDC KR-1, CDC Redberry, CDC Robin, CN 105604, ILL 213, PI 612875, ITACA
Phenomics
Key Measurements
CIE L*, a* and b* colour scores
Experimental Design
Experimental Site Locations
  • Sutherland, Saskatchewan, Canada
  • Rosthern, Saskatchewan, Canada
  • Osimo, Ancona, Italy
Timepoints
2022
Experimental Design

  • Field trials were arranged in a Randomized Complete Block Design (RCBD) with seed for this experiment being taken from a subset of the site-years of the full experiment. This resulted in four (Sutherland2022, Rosthern2022) and three (Osimon2022) repetitions of each lentil genotype. See the original experiment for more details: Lentil Red cotyledon intensity: Lentil germplasm over 8 site-years in 2 macroenvironments from 2022-2023 using BELT.

  • There were 3 technical replications per colorimeter done on every sample when measuring the colour values. All samples were measured with each of 1) HunterLab Mini XE Plus, 2) Nix Spectro 2 with illuminant C, 3) Nix Spectro 2 with illuminant D50.

  • Seeds from all replications within a site-year where combined for each genotype. Whole seeds were collected at maturity and dried at 45°C until a moisture content of <13% was reached. Clean, dried seeds were dehulled using a Satake TM05C huller and finely ground using a Udy cyclone sample mill through a #60 mesh size (0.25 mm).

Attribution
Researchers
Data Collector
Data Custodian
Data Curator
Associated Datasets

Pressed Flour CIE L*, a*, b* scores (Excel, TSV)

This dataset consists of the CIE L*, a*, and b* scores for dry pressed flour samples. Flour samples were obtained from seeds grown in the Sutherland2022, Rosthern2022 and Osimo2022 site-years of this linked experiment: Lentil Red cotyledon intensity: Lentil germplasm over 8 site-years in 2 macroenvironments from 2022-2023 using BELT. Samples were measured with the HunterLab Mini XE Plus and the Nix Spectro 2 with illuminants D50 and C (referred to as "HunterLab", "Nix C" and "Nix D50" respectively). All samples were measured 3 times (technical replicates) and there were 4 biological/field replicates for Sutherland2022/Rosthern2022 samples and 3 biological/field replicates for Osimo2022. The field plot IDs between this file and those on the linked experiment match.

Funding
Research Organization
Funding Grant
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
  • Kirstin E Bett
  • Albert Vandenberg
University of Saskatchewan

2019-2023

Mitacs Accelerate Grant (IT29802)
Kirstin E Bett
University of Saskatchewan

2022-2024