Lentils are a good dietary source of essential minerals for the proper functioning of the human body. We evaluated 34 cultivars and elite lentil lines representing the breadth of the Canadian breeding program. Trials were established in 10 site-years across Saskatchewan. Concentrations of 27 minerals were quantified with an inductive coupled argon plasma emission spectrometer in whole and dehulled lentil seeds. Lithium (Li), vanadium (V), chromium (Cr), cobalt (Co), arsenic (As), silver (Ag), cadmium (Cd), tin (Sn), lanthanum (La), mercury (Hg), and lead (Pb) had concentrations below the quantification limit and were excluded from further analysis. The effects of site year, tissue type (whole and dehulled), and lentil genotypes were analyzed using a mixed model. Mineral concentrations of iron (Fe), zinc (Zn), magnesium (Mg), sodium (Na), potassium (K), phosphorus (P), sulfur (S), manganese (Mn), copper (Cu), molybdenum (Mo), boron (B), nickel (Ni), selenium (Se), aluminum (Al), and barium (Ba) was different among genotypes and across environments. Most minerals except Cu had significantly different concentrations between whole and dehulled seeds. Ca, Fe, Mg, Mn, B, Al, and Ba were more concentrated in whole seeds, while K, S, and Ni were higher in dehulled seeds. Among the 34 genotypes, CDC Impala had the highest Fe, Zn, K, P, S, and Cu concentrations. Lentil genotypes with a higher composition of several minerals could be a starting point for enhancing mineral composition in lentils.