B vitamin quantification: diverse Lens spp. seed tissues using UPLC/MRM MS performed in 2022

Overview
Objectives

Characterize the nutritional and food functionality characteristics of a diverse set of lentil germplasm to understand their B vitamin profile.

Parent Research Study
B vitamin quantification in wild and cultivated lentil seed tissues using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry
Experiment Summary

A group of 37 globally diverse lentil genotypes, including thirty-four cultivars and elite lines and 3 wild accessions, were evaluated to assess the variation on B vitamin concentration cross genotypes and seed tissue types (seed coat, cotyledon and whole seed). This will be the groundwork for dissecting the genetics of B vitamins concentration in lentils.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
Lens culinaris
Germplasm Collection
EVOLVES B vitamin diversity panel
Phenomics
Key Measurements
  • Per B-vitamin: Concentration in Seed Coat (ug/g)
  • Per B-vitamin: Concentration in Cotyledon (ug/g)
Experimental Design
Experimental Site Locations
  • Sutherland, Saskatchewan, Canada
  • Preston, Saskatchewan, Canada
Timepoints
  • 2018
  • 2020
Biochemical Technique
ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry (UPLC/MRM MS)
Experimental Design

  • Whole seeds for each genotype were air dried at 45°C until a moisture <13% was reached. Seeds from each plot were then divided into two groups 1) seed coats, 2) cotyledons. Seeds were separated into cotyledons and seed coats using a Satake huller TM05C(2).

  • Seed samples were ground to a fine powder using an Udy grinder. Equal amounts powdered sample per plot for a single genotype were combined for each of 1) seed coats, 2) cotyledons. These samples were stored at -80°C until analysis.

  • B vitamins were extracted from these samples and then measured using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry (UPLC-SRM MS) analysis with resulting values being expressed in micrograms (ug) of B vitamin per gram (g) dry weight of initial powdered sample.

Attribution
Researchers
Data Collector
Jeremy Marshall
Data Custodian
Data Curator
Ruobin Liu
Publication(s)
Marshall J, Vargas A, Bett K. B vitamin quantification in lentil seed tissues using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry.. Food chemistry. 2023 Jul 17; 136922.
Associated Datasets

LDP B Vitamin Concentration Dataset (XLSX, TSV)

This data consists of the concentration (ug B vitamin per gram dry weight of initial powdered sample) for seven B vitamins. Samples consist of 2 site-years, 6 Lentil genotypes and either seed coat or cotyledons. In 2018 the samples were per field plot but in 2020 the field plots per genotype were pooled into a single sample. The B vitamins were measured using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry (UPLC-SRM MS).

Funding
Research Organization
University of Saskatchewan
Funding Grant
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
  • Kirstin E Bett
  • Albert Vandenberg
University of Saskatchewan

2019-2023