Investigating interspecific lentil germplasm: Near-Infrared spectorscopy for protein and amino acid contents and quantitative trait loci analyses

Objectives

  • Investigate the effects of crossing with wild lentil on seed protein and amino acid contents.

  • Quantify the amount of total protein and 18 specific amino acids in the RILs developed from an interspecific cross using near-infrared (NIR) spectroscopy.

  • Extend the model developed by Jiayi Hang in Protein characterization: LDP 2020-2021 using NIRS for use with single Lentil seeds.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
  • Lens culinaris
  • Lens orientalis
Germplasm Collection
LR-68 (L. orientalis IG 72643 x L. culinaris 3339-3)
Executive Summary

This study aimed to investigate the effects of crossing with wild lentil on seed protein and amino acid contents. An interspecific recombinant inbred line (RIL) population, LR-68, generated from a cross between Lens culinaris and Lens orientalis was grown in four site-years in Saskatchewan, Canada. LR-68 was evaluated for protein and 18 amino acid contents using near-infrared (NIR) spectroscopy. Quantitative trait loci (QTL) analyses were then conducted to identify regions of the genome associated with protein and amino acid contents using the LR-68 (Zhe Cao et al., 2023) genetic linkage map. LR-68 exhibited significant variation for protein and amino acid content, with protein content ranging from 24.9 to 34.9% on a dry basis. Genetic analyses revealed three QTL dense regions, with QTL for protein and multiple amino acids present at the same loci. At two of the three QTL dense regions, genotypes containing the allele from the wild parent, L. orientalis, had significantly higher protein and amino acid contents. Selecting for increased protein content when crossing with L. orientalis will also increase individual amino acid contents.

Attribution
The following researchers and their organizations were involved in this work and should be credited for their role in any resulting or related publications.
Data Custodian
Collaborator
  • Eric von Wettberg (University of Vermont)
  • Janitha Wanasundara (AAFC, Saskatoon Research Centre)
  • James House (University of Manitoba)
  • Scott Noble (University of Saskatchewan)
Data Curator
Research Organization
Publication
Jendrasheske N (2024) Investigating Interspecific Lentil Germplasm: Near-Infrared Spectroscopy for Protein and Amino Acid Contents and Quantitative Trait Loci Analyses [Masters Thesis, University of Saskatchewan]. Harvest. https://hdl.handle.net/10388/15982.
Experiments
Protein profile differences: LR-68 2022-2023 using NIRS
Data Collector
Noah Jendrasheske
Timepoints
  • Summer 2022
  • Summer 2021
Key Measurements
  • Total Protein Content (g)
  • Total protein percent composition on a dry matter basis (%)
  • Amino Acid specific content (g) for each of 18 amino acids
  • Amino Acid specific percent composition on a dry matter basis (%) for each of 18 amino acids
Objectives
  • Quantify the amount of total protein and 18 specific amino acids in the RILs developed from an interspecific cross using near-infrared (NIR) spectroscopy.

  • Extend the model developed by Jiayi Hang in Protein characterization: LDP 2020-2021 using NIRS for use with single Lentil seeds.

Associated Datasets

LR-68 Protein Percent Composition NIRS Dataset (XLSX, TSV)

This dataset consists of the total protein percent composition on a dry matter basis and amino acid specific percent composition on a dry matter basis for each of 18 amino acids. The protein compositions were determined using near-infrared reflectance spectroscopy (NIRS) models.

Grant Activity
EVOLVES Activity 2- Genomics and phenomics of nutritional and food functionality characteristics of lentil
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
  • Kirstin E Bett
  • Albert Vandenberg
University of Saskatchewan

2019-2023