Assessment of Optical Coherence Tomography as a non-destructive method for seed coat thickness measurement

Objectives
  • Explore using optical coherence tomography (OCT) as a non-destructive and efficient measurement of seed coat thickness.

  • Dissect any potential correlations between seed coat thickness and biological characteristics such as imbibition, dehulling, and cooking time.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
  • Lens culinaris
  • Lens orientalis
Germplasm Collection
LR-68 (L. orientalis IG 72643 x L. culinaris 3339-3)
Executive Summary

With the aid of the imaging technique known as optical coherence tomography (OCT), optical scattering media (e.g., biological tissue) can be imaged in two and three dimensions with micrometer-resolution. OCT is utilized in industrial non-destructive testing (NDT) and medical imaging. Similarly, OCT can be applied in agriculture and different biological tissues, such as seeds.

This project provides an opportunity to measure observable traits in several types of seeds, and to reach broad information about the seed. Seed characteristics such as imbibition, dehulling, and cooking time might have a correlation with observable seed traits like seedcoat thickness, shape of seed, roundness, surface roughness. To measure these traits, researchers have used different methods which are typically destructive, and time-consuming. In this project, further testing will be conducted, using Optical Coherence Tomography (OCT), to measure those traits and find a potential correlation between biological and physical characteristics by interpretation of OCT images.

Attribution
The following researchers and their organizations were involved in this work and should be credited for their role in any resulting or related publications.
Data Custodian
Kirstin E Bett
Data Curator
Lacey-Anne Sanderson
Research Organization
Experiments
Grant Activity
Title
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
Data Custodian
  • Kirstin E Bett
  • Albert Vandenberg
Research Organization
Funding Range

2019-2023