The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples.

Title

The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples.

Publication Type
Journal Article
Series Name
Plant methods
Volume
16
Publication Year
2020
Page Numbers
49
DOI
10.1186/s13007-020-00591-8
Journal Abbreviation
Plant Methods
Publication Date
2020
Unique Local Identifier

Halcro K, McNabb K, Lockinger A, Socquet-Juglard D, Bett KE, Noble SD. The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples.. Plant methods. 2020; 16:49.

Citation
Halcro K, McNabb K, Lockinger A, Socquet-Juglard D, Bett KE, Noble SD. The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples.. Plant methods. 2020; 16:49.
ISSN
1746-4811
Language Abbr
eng
Publication Type
Journal Article
Publication Model
Electronic-eCollection
Authors

Halcro K, McNabb K, Lockinger A, Socquet-Juglard D, Bett KE, Noble SD

Language
English
Elocation
10.1186/s13007-020-00591-8
Journal Country
England
Abstract

Background
Quantitative and qualitative assessment of visual and morphological traits of seed is slow and imprecise with potential for bias to be introduced when gathered with handheld tools. Colour, size and shape traits can be acquired from properly calibrated seed images. New automated tools were requested to improve data acquisition efficacy with an emphasis on developing research workflows.

Results
A portable imaging system (BELT) supported by image acquisition and analysis software (phenoSEED) was created for small-seed optical analysis. Lentil (Lens culinaris L.) phenotyping was used as the primary test case. Seeds were loaded into the system and all seeds in a sample were automatically individually imaged to acquire top and side views as they passed through an imaging chamber. A Python analysis script applied a colour calibration and extracted quantifiable traits of seed colour, size and shape. Extraction of lentil seed coat patterning was implemented to further describe the seed coat. The use of this device was forecasted to eliminate operator biases, increase the rate of acquisition of traits, and capture qualitative information about traits that have been historically analyzed by eye.

Conclusions
Increased precision and higher rates of data acquisition compared to traditional techniques will help to extract larger datasets and explore more research questions. The system presented is available as an open-source project for academic and non-commercial use.

Database Reference Annotations
Is Obsolete
False