Carotenoid Concentrations: LR-68 2018 using LCMS

Overview
Objectives

Measure the carotenoid concentration in a RIL population segregating for cotyledon colour to determine if there is a link between red-yellow cotyledon colour and carotenoid concentration.

Parent Research Study
Using RNAseq and Structural variant data to futher explore genetic control of red-yellow cotyledon colour
Experiment Summary

Seeds from a field trial at Sutherland in 2018 for an interspecific recombinant inbred line population of 120 RILs (LR-68, F8-derived) were used for carotenoid quantification. All carotenoid concentrations were determined using liquid chromatography/mass spectrometry (LC/MS) and recorded in micro grams per gram (µg/g). In addition, the cotyledon color of dehulled seeds was quantified using a Nix Spectro 2 device and data decomposed into CIELAB L* a* b* colour space values.

Germplasm
Germplasm Genus
Lens
Germplasm Scientific Name
  • Lens culinaris
  • Lens orientalis
Germplasm Collection
LR-68 (Lens orientalis IG 72643 x Lens culinaris 3339-3)
Phenomics
Key Measurements
  • carotenoid concentration (µg/g)
  • CIE L*, a* and b* colour scores
Experimental Design
Experimental Site Locations
Sutherland, Saskatchewan, Canada
Timepoints
2018
Biochemical Technique
liquid chromatography/mass spectrometry (LC/MS)
Attribution
Researchers
Data Collector
Jeremy Marshall
Data Custodian
Data Curator
Ruobin Liu
Associated Datasets

LR-68 Carotenoid Dataset (XLSX, TSV)

This dataset consists of the concentration (ug compound per gram dry weight of initial powdered sample) for four carotenoids, as well as the total carotenoid. Samples consist of 1 site-year and 117 recombinant inbred lines.

Funding
Research Organization
University of Saskatchewan
Funding Grant
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
  • Kirstin E Bett
  • Albert Vandenberg
University of Saskatchewan

2019-2023