Free SH Group and Soluble Protein: LDP 2022 using BCA (Bicinchoninic acid) protein assay
Overview
Parent Research Study
Experiment Summary
Subsamples of different lentil varieties for this project were collected from previous EVOLVES work completed at the University of Manitoba, Winnipeg to include high, mean, and low protein and amino acid content (total of 18 and cysteine) (Hang et al., 2022). The soluble protein content of lentil flour extracts, free sulfhydryl (thiol, -SH) groups was determined. Soluble protein content will be determined by BCA (Bicinchoninic acid) protein assay following the test tube procedure in Thermo Scientific™ Pierce™ Kit for colorimetric detection and quantification of protein which will be expressed as BSA (Bovine Serum Albumin) equivalents. Free sulfhydryl groups will be determined according to Mession et al (2015, Food Hydrocoll, 46, 233)
Germplasm
| Germplasm Genus |
Lens
|
|---|---|
| Germplasm Scientific Name |
Lens culinaris
|
| Germplasm Collection |
Crimson, Eston, ILL 5888, ILL 4164
|
Phenomics
Key Measurements
- Free sulfhydryl group per mL (µM/mL)
- Free SH group concentration (mg soluble protein/mL basis)
Experimental Design
| Experimental Site Locations |
University of Manitoba, Winnipeg, MB, Canada
|
|---|---|
| Timepoints |
|
| Biochemical Technique |
BCA (Bicinchoninic acid) protein assay
|
Attribution
Researchers
| Data Collector | |
|---|---|
| Data Custodian |
|
| Data Curator |
Associated Datasets
Dataset
Experiment Results (Excel, TSV)
The results of five experiments performed to determine the free sulfhydryl group concentration for the four genotypes: Crimson, Eston, ILL 5888, ILL 4164. Soluble protein content was determined by BCA (Bicinchoninic acid) protein assay following the test tube procedure in Thermo Scientific™ Pierce™ Kit for colorimetric detection and quantification of protein which will be expressed as BSA (Bovine Serum Albumin) equivalents. Free sulfhydryl groups were determined according to Mession et al (2015, Food Hydrocoll, 46, 233)
Funding
Research Organization
Funding Grant
Grant Logo
Title
EVOLVES: Enhancing the Value of Lentil Variation for Ecosystem Survival
Data Custodian
- Kirstin E Bett
- Albert Vandenberg
Research Organization
Funding Range
2019-2023