Lens orientalis: BGE 016880 Genome Assembly 1.0
Overview
| Assembly Genome Size |
3.75 Gb
|
|---|---|
| Number Of Scaffolds |
696
|
| Scaffold N50 length |
504 Mb
|
| Contig N50 Length |
2.316 Mb
|
Description
This assembly of BGE 016880 (Lens orientalis) was constructed with long-read data (50x Oxford nanopore reads). There were 3 706 total contigs with the longest contig spanning 11.4 Mb. The contiguity of the assembly was further validated and improved using HiC data and a genetic map (LR-89; BGE 016880 x IG 72529 intraspecific RIL). The finished assembly is 3.75 Gb arranged in 7 pseudo-molecules and 689 unplaced unitigs (98.6% anchored).
Methodology
| Genus |
Lens
|
|---|---|
| Scientific Name |
Lens orientalis
|
| Data Source Version |
Sequencing 2021
|
| Data Source Name |
BGE 016880
|
| Abbreviation |
Lor.1WPS
|
|---|---|
| Program, Pipeline, Workflow or Method Name |
GenomeFocus long read genome assembly workflow for large and highly repetitive genomes
|
| Program Version |
Oct 2021
|
Analysis Method
Using the GenomeFocus workflow, long reads were assembled with smartdenovo and polished with racon. Due to the high degree of telomere tethering observed in Lens species, a genetic map (LR-89) was used to form the initial clusters for HiC data incorporation. ALLHiC is then used to order and orient contigs within each pseudomolecule cluster. Any corrections and a final manual check are made with Juicebox.
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Data Release
This genome is available in pre-release form below. Specifically, you will receive a compressed archive of the genome assembly and associated files including a README with basic information.
Please contact Dr. Kirstin Bett for permission to publish an analysis using this genome assembly. Once permission is obtained, you can cite this assembly as:
Ramsay L, Koh K, Bett KE (2022 Jun 23) Lens orientalis: BGE 016880 genome assembly (Lor.1WPS, version 1.0) [dataset]. University of Saskatchewan. Retrieved from https://knowpulse.usask.ca/genome-assembly/Lor.1WPS
Genome Assembly: Lor.1WPS.zip
- File Size: 1.007 GB
- Md5 Checksum: 0e779e3a227f42166b44d27ebfbc468a
- Last updated: Sep 2, 2026