Development of improved lentil cultivars well-adapted to the local environment is an on-going process in the breeding program and is critical for long-term genetic gain. Recent climate instability adds another layer of complexity to breeding efforts. Continued genetic improvement of lentil will, therefore, involve the introduction of new alleles that extend beyond the existing adapted pool of germplasm. Our goal in AGILE is to enhance the productivity and quality of Canadian lentils by expediting the expansion of genetic diversity of the Canadian lentil germplasm base with the use of genomic technologies.
<p>A diverse collection of lentil accessions is being phenotyped for days to flower and screened against potential flowering time genes Identified by other researcher groups. In addition to the confirmation and the development of markers useful for the prediction of flowering time in northern temperate (Sask.) conditions, the identification of other candidate flowering time genes are goals of this project.</p>
Stone seeds, which are seeds that do not absorb water, are considered a negative seed quality characteristic because they need to be removed before commercial processing. A high physical dormancy at the end of seed development is found to be the cause of this issue, but it is not known how or when it develops. This project will focus on attempting to determine when the seeds begin to develop physical dormancy, and also how to avoid hard seededness through harvest times.
This is an international project funded by the Global Crop Diversity Trust aimed at evaluating cultivated x wild lentil introgression lines for multiple traits in multiple environments.
As production of the dry bean is moving towards short season growing regions such as Alberta and Saskatchewan, it is becoming increasingly important to find a way to develop abiotic stress tolerances for the dry bean. Through the incorporation of genes from other species, the stress tolerance capabilities of the dry bean will increase, making it less sensitive to its surrounding climate. The tepary bean was decided upon as the best genetic donor for improvement to the dry bean, and is now being evaluated in Saskatchewan and its international partners.
Our approach to sequencing the lentil genome is two-fold. First, we are generating a high quality draft genome for a single lentil genotype (CDC Redberry), including bulk sequencing, assembly of chromosomal ‘pseudomolecules’, and gene prediction and annotation. Secondly, we are re-sequencing various lentil accessions from around the globe to reveal the breath of genetic potential present in our germplasm resources. The outcome will give us i) an understanding of how modern breeding has re-shaped the lentil genome, ii) identification of genes and genomic interval that control agronomic traits, and iii) discovery of many genetic polymorphisms for future marker development, that together will add considerable resources to the breeder’s toolbox for lentil genetic improvement. More importantly, the results of this project will allow us to leverage knowledge of important trait based on conservation of genome-based features with other legume crops (such as Medicago and chickpea).
Lentils are seen as a source for essential vitamins and minerals for human nutrition, but due to the high anti-nutritional factors of raffinose family oligosaccharides the consumption of lentils are being limited. Other methods to lower the levels of these RFOs are costly, and that is why an alternative strategy to develop varieties of lentil with lower levels is being implemented.
This group is involved in a wide range of biotechnology projects that accelerate the legume breeding process. Double-haploid technology has been achieved in both chickpea and field pea by the CDC group in collaboration with colleagues in France and Australia. Efforts are underway to adapt this technology to lentil. Improving efficiency and integrating these techniques into routine breeding programs to enhance genetic gain are important long-term goals.
The objectives of this study are to determine the effect of genotype and environment on iron bioavailability in a set of five pea varieties differing in phytate concentration using the Caco-2 mammalian cell bioassay, to determine whether iron bioavailability in field pea is heritable by evaluating recombinant inbred lines differing in phytate concentration using the Caco-2 mammalian cell bioassay, and to determine the effect of the pea low phytate trait on chicken performance and iron bioavailability in chicken.
A number of KASP markers were developed based on the genotypes identified under the Lentil 454 Sequencing Project. An initial set were used for validation of the SNP calling before developing the Illumina Golden Gate Assay (Lc1536). An additional 350 KASP primers were then designed for the SNPs that were successfully mapped using data from the GoldenGate array (see Fedoruk et al. 2013).