Field assessment of outcrossing from transgenic pea (Pisum sativum L.) plants

Overview
TitleField assessment of outcrossing from transgenic pea (Pisum sativum L.) plants
AuthorsPolowic Patricia L, Vandenberg Albert, Mahon John D
TypeComparative Study
Volume11
Issue5
ISSN0962-8819
Journal AbbreviationTransgenic Res.
Journal CountryNetherlands
LanguageEnglish
Language Abbreng
pISSN0962-8819
Publication Date2002 Oct
Publication ModelPrint

Abstract

<p>The frequency of outcrossing from a transgenic line of peas into three cultivars ('Carneval', 'Montana', 'Tipu') was studied in the field in 1997 and 1999. Two dominant traits, normal leaf form and a highly-expressed beta-glucuronidase (gusA) gene, were used as markers of pollen transfer. Because of heterogeneity in the commercial seed sources, leaf form alone was unreliable for assessing pollen migration into 'trap' plants. Of approximately 9000 offspring tested, only five plants scored positive for the presence of both markers. All five were located in 'trap' plots situated near the transgenic line. This represents a mean outcrossing rate of 0.07%.</p>

Citation

Polowic PL, Vandenberg A, Mahon JD. Field assessment of outcrossing from transgenic pea (Pisum sativum L.) plants. Transgenic research. 2002 Oct; 11(5):515-9.

Related Species
Related Species: 

Pea (Pisum sativum L.) is one of the first domesticated crops, and was the model crop for the foundational genetic studies by Gregor Mendel, which he first reported in 1865. Pea is grown in most temperate regions of the world with annual production over the past decade of 10-12 million tonnes of field pea and 14-17 million tonnes of vegetable pea. Pea belongs to the Leguminosae family and consists of two species, P. fulvum and P. sativum with several ‘wild’ subspecies of P. sativum. Canada is the leading producer and exporter of field pea in the world. Saskatchewan is the leading province in pea production followed by Alberta and Manitoba. ... [more]

 
Cross References
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DatabaseAccession
PMID: PubMedPMID:12437082
Research Area
Research Area: 
Physiology

The objectives of the Pulse Research Group Physiology Program is to investigate whole plant and field responses of crops, particularly pulse crops, to nutrient, water and weather.  To understand and improve yield formation in pulse crops in a warming climate.  To investigate and improve nitrogen ... [more]