Lentil UC Davis COS Genotyping

Overview
2009

A set of 1107 legume cross species orthologous sequences (COS) were amplified   from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815).  Sequences were aligned and SNPs identified.  A subset of 110 KASP assays were designed for use in L. culinaris.  An Illumina GoldenGate array of 768 SNPs was designed for use in L. ervoides or interspecies hybrid populations between Lc and Le.

A map of L.ervoides, consisting of 377 SNP markers spanning seven linkage groups was developed and used to compare the genetic structure of Le with Medicago.  There was extensive collinearity between the 2 genomes.

Properties
Additional information about this project:
Property NameValue
TypeResearch Experiment
Research Area
Breeding & Genetics

Plant breeding is the art and science of changing the traits of plants in order to produce desired characteristics. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques. ... [more]

 
Related Species

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]

 

Lens ervoides is a wild relative of Lens culinaris with semi-hastate or lanceolate stipules. L. ervoides can be distinguished from other wild Lentil species by its smaller leaves, calyx teath, pods and seeds1. L. ervoides is often found in shady or partially shady niches, such as among bushes or under trees, with stony soils1. Unlike other wild lentil species, L. ervoides is rarely found in mixed stands with other wild lentils1. Recent sequence analysis indicates that Lens ervoides is in the tertiary gene pool of L. culinaris2 ... [more]

 
Sequences, Variants & Markers
Data Type Number of Sequences Number of Variants Number of Markers
Sanger Sequencing 9,572*
SNP 7,241*