Lens culinaris

Overview
GenusLens
Speciesculinaris
Common NameCultivated Lentil
AbbreviationL.culinaris

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod.

Breeding at the University of Saskatchewan

Market Classes: Large Green, Medium Green, Small Green, Small Red, Extra Small Red, as well as, a Few Minor Specialty Classes.

Breeding Objectives: High Yield, Lodging Tolerance, Appropriate Size, Shape, Seed Coat Colour & Quality, and Resistance to Ascochyta Blight, Anthracnose, Stemphylium Blight & Botrytis .

Germplasm Data
The following germplasm data is currently available:
Stock TypeCount
Single Cross2,321
Single Cross2,321
Triple Cross1,498
Triple Cross1,498
Individual1,025
Individual1,025
DNA472
DNA472
Multiple Cross365
Multiple Cross365
variety358
variety358
Backcross243
Backcross243
Double Cross126
Double Cross126
Population58
Population58
Sequence & Variant Data
The following sequence and variant data are currently present:
Feature TypeCount
marker56,562
marker56,562
SNP52,183
SNP52,183
contig28,939
contig28,939
EST9,513
EST9,513
MNP1,543
MNP1,543
read_pair1,206
read_pair1,206
indel789
indel789
Nutritional Facts

Lentils, raw (dry weight)

Energy
343.00
kcal
Carbohydrate, by difference
60.08
g
Fiber, total dietary
30.50
g
Sugars, total
2.03
g
Projects
2009
Lentil is an economically important pulse crop for Canada produced mainly for the export market. In conventional breeding programs, several segregating generations must be grown in order to reach a certain level of homozygosity that allows the selection of traits of interest. In contrast, double-haploid (DH) technology produces instant homozygosity and thus can significantly reduce the time required for developing new varieties. The efficiency of the lentil breeding program will also be improved through the reduction in the population size required for screening.
2009
Colletotrichum truncatum is a pathogen of several leguminous plant species where it causes a disease called anthracnose. Host specialization, infertility and genetic differentiation among isolates from different hosts have been demonstrated. In the population of C. truncatum from lentil two races were described. Detailed histological studies of the infection process by isolates of both races have revealed quantitative rather than qualitative differences.
2009
Lentil anthracnose is currently the most important lentil disease in Saskatchewan. In a project about to be completed on molecular aspects of this pathogen, we generated an extensive library of genes activated by the anthracnose fungus and by lentil during the infection process. In order to fully benefit from the investment made in this NSERC-CRD, we received one-year funding to confirm the function of a number of genes in the anthracnose fungus, Colletotrichum truncatum, that we have identified and believe to be involved in virulence of the fungus.
2009
Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
Ninety-six USDA lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
Ninety-six USDA lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
A set of 1107 legume cross species orthologous sequences (COS) were amplified from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815). Sequences were aligned and SNPs identified. A subset of 110 KASP assays were designed for use in L. culinaris. An Illumina GoldenGate array of 768 SNPs was designed for use in L. ervoides or interspecies hybrid populations between Lc and Le.
2008 to 2009
Mixture of eight cultivars with varying seed phenotypes: Indian Head, Commando, CDC LeMay, CDC Robin, and breeding lines 1899T-50 and 1788-4 (CDC, Univ. Saskatchewan, Saskatoon, Canada) All developmental stages of seeds and very young fertilized pods were harvested from mature plants, and divided into the following lots: very young fertilized ovaries, young ovules, enlarging seeds, cotyledons of fully filled seed, seed coats of fully filled seeds. cDNA library was made from a mixture of equal amounts of mRNA extracted from each of the above tissues.

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